Sednterp can interpolate the viscosity of a buffer from viscosity table data in the phyconst database. 47 48) The viscosity is dependent on both the temperature and the solution composition. 2 14 17) Excellent reviews are available describing methods for determining viscosity and for interpreting viscosity data.
Therefore, solvent viscosity will affect both sedimentation velocity and the time needed to reach equilibrium. For a fixed amount of energy, the greater the solvent's viscosity, the slower the protein will move.
In sedimentation, the flow of interest is the movement of the protein through the solvent. Viscosity is a measure of the energy dissipated in a fluid in order to maintain flow.
